Since 1989, there have been 12 laboratories—lovingly nicknamed the dirty dozen—that are suggested curricula for ap biology classes they span the globe in their topics and their techniques: from a behavior lab involving detailed visual observations to a biotechnology lab involving electrophoresis equipment with an extensive protocol. Lab 6a - bacterial transformation & ampicillin resistance introduction: bacterial transformation occurs when a bacterial cell takes up foreign dna and incorporates it into its own dna. Developed with master ap teachers, bio-rad's pglo transformation and inquiry kit for ap biology includes five investigative labs in one kit to provide teachers with the tools and resources they need to guide students through the process of scientific discovery while developing their skills and gaining the knowledge that will help prepare them for success on the ap exam. Write your lab group's name or initials on each tube using a disposable pipette, add 250 µl of 50mm cacl 2 solution to each tube (+ and -) and immediately place them both on ice use a sterile plastic loop to transfer one or two 3-mm bacterial colonies or an equivalent amount of smaller colonies from the streak plate to the. Ap biology homework read pglo plates and answer questions from lab for solve unknown sucrose molarity values and work on lab write-up ap lab#1 due.
Introduction our ap biology lab activities are designed to provide a wide variety of experiences they will fall into two general categories: observational or skill labs, and experimental labs observational labs will mainly involve watching or observing natural phenomena occur or performing some scientific technique, while experimental labs will involve science process skills. Paul andersen details the first 7 of 13 labs in the ap biology curriculum the following topics are all covered: artificial selection, hardy-weinberg equilibrium, comparing dna using blast. Huber 1 mara huber 7 october 2010 ap biology name of partners - maggie coakley, ashtin massie, natasha meyer, erica westwater lab one - diffusion and osmosis purpose - the purpose of this lab is to investigate the processes of diffusion and osmosis in a model membrane system and the effect of solute concentration on water potential as it relates to living plant tissues. © copyright nc state university 2004 sponsored and funded by national science foundation (due-9950405 and due-0231086.
Biotechnology bacterial transformation lab: the effects of pglo dna on e coli method introduction bacteria transformation is the process of a bacterium absorbing and integrating naked dna located on the surface of their membrane. Summary: the pglo bacterial transformation lab is based off the molecular biology ap biology lab involving transformationthe progression from dna- rna- protein- trait is shown with the bacteria e coli. Bio-rad's unique pglo plasmid encodes the gene for gfp and a gene for resistance to the antibiotic ampicillin pglo also incorporates a special gene regulation system, which can be used to control expression of the fluorescent protein in transformed cells.
The lab groups together, there was a major difference in the response of flies to the sugars and to saccharin (table 1) when all the sugars were considered together, this difference. Pglo transformation lab answer keypdf free pdf download now source #2: pglo transformation lab answer keypdf free pdf download 26,600 results any time. Mary will lead participants through the new ap biology labs, some low-cost alternatives to the new labs, and suggest ways to make teachers' existing labs more inquiry-based, in line with the new ap biology framework.
The write-up for the pglo lab should be a useful summary of the lab bacterial transformation with (pglo plasmid) in accelerated biology test protein synthesis, gene regulation. Ap biology lab six a and b: dna fingerprinting and bacterial transformation a single colony of bacteria was picked up from the starter plate and transferred to. Name: _____ ap biology - lab 16 page 5 of 15 the dna code of the pglo plasmid has been engineered to incorporate aspects of the. Introduction in this lab, the goal was to transform the bacteria e-coli to glow in the dark (or under a black light) four plates were set up with agar in them for the bacteria to feed on and grow.
They don't write up the genetics of drosophila lab or the hardy-weinberg lab we learned the lesson well the year of the first molecular biology lab essay question we did that lab last—just before the exam. Transitioned from the ap biology lab manual (2001) t144 investigation 8 bacterial plasmid-based genetic transformation, enables students to manipulate genetic. Acids & bases lab write up lab 2 introduction there are many acids and bases all around us an acid is known as a hydrogen ion donor (h¯), and a base is known as a hydrogen ion acceptor (h⁺. Can anyone in ap bio, or anyone that remembers ap bio accurately, explain the pglo lab for me i've already done the prac, but i'm trying to write up conclusions i know what happened and why the genetic transformation occured, but.
Ap biology with mrs heaton ap biology 2017-2018 rubric for lab write up pglo lab transformation ppt. A lab report composed in 2008 regarding the transformation lab for the collegeboard's ap biology program. Lab component the laboratory experience is extremely important in the ap biology course and is used to emphasize that biology and science is a process, which involves development and testing of a hypothesis, collection, analysis. Computer 6a advanced biology with vernier 6a - 1 pglo™ bacterial transformation introduction to transformation in this lab, you will perform a procedure known as genetic transformation.