How enzymes work in different environments by sarah smith biology1111 october 20, 2011 lab partner: nellie greer abstract peroxidase is an enzyme found in potatoes that catalyzes the breakdown of hydrogen peroxide, h2o2, into o2 gas and water. In this lab you will determine the effect of temperature and ph on the activity of the peroxidase enzyme of turnips peroxidase enzymes are found in many organisms and typically catalyze the conversion of hydrogen peroxide (h 2 o 2 ) to water (h 2 o. The main enzyme for this lab, peroxidase, is found in many different forms, with optimum phs ranging from 4 to 11 depending on the source and optimum temperatures varying from 10 to 70°c. In this lab we explore an enzymes activity and how it can be affected by changes to its environment an enzyme is a protein and is a catalyst to chemical reactions (raven, 2011.
Enzyme activity guided inquiry lab turnip peroxidase continued 2 21 inn cientiic inc a its eserved safety precautions the guaiacol solution contains isopropyl rubbing alcohol (70%) and is a flammable liquid. Name: _____ ap biology - lab 05 page 3 of 7 in this experiment, you will study the enzyme peroxidase obtained from turnips.
Transcript of peroxidase lab report the effect of ph on enzyme activity hypothesis the ph will have an effect on enzyme activity as seen through the amount of water displaced, the enzymes in the optimal ph environment of 6 will be the most active, displacing the most water, followed by the basic environment of 9, and the acidic environment of. Peroxidase enzyme biology lab report (half way finished) how enzymes work in different environments by sarah smith biology1111 october 20, 2011 lab partner: nellie greer abstract peroxidase is an enzyme found in potatoes that catalyzes the breakdown of hydrogen peroxide, h2o2, into o2 gas and water. The effect of temperature on the action of peroxidase enzyme - the effect of temperature on the action of peroxidase enzyme aim to find the effect of temperature on an enzyme in this case peroxidase, by studying it decomposing hydrogen peroxide.
Test how peroxidase enzyme is affected by factors such as enzyme concentration, ph, and temperature measure the effects using guaiacol, a colorless compound with formula c7h8o2 when guaiacol is oxidized, it converts to a dark orange compound called tetraguaiacol. Peroxidase is an enzyme found in a wide variety of organisms, from plants to humans to bacteria its function is to break down hydrogen peroxide (h 2 o 2 ), which is one of the toxins produced as a byproduct of using oxygen for respiration. The amount of 02 gas produced was then measured and recorded the result was that the higher ph produced more gas, followed by medium ph, then low ph the enzymes were more active in the ph of about 10.
Knowledge and understanding (concept analysis): enzymes are made of protein, depending on the structure of the amino acid, and the hydrogen and ionic bonds is what makes the difference between the two enzymes (catalase and peroxidase. In this lab, you will study an enzyme that is found in the cells of many living tissues the name of the enzyme is catalase it speeds up a reaction which breaks down hydrogen peroxide, a toxic chemical, into 2 harmless substances--water and oxygen. We will write a custom essay sample on peroxidase lab report specifically for you for only $1638 $139/page peroxidase enzyme biology lab report (half way finished.
Conclusion: this lab was helpful in showing us how enzyme catalysis really happens in biology to get better results, we should have done this lab over again, but time did not allow us to do so the results we got, however, were roughly to be expected. Emily analysis essays essay driving up the coast research papers enzyme lab report lactase is an enzyme that works on lactose, a sugar lactase is an enzyme that works on lactose, a sugar this lab will examine the specificity of an enzyme (lactase) to a specific substrate (lactose. Lab 6: enzyme kinetics biology 171l 3 the reactions catalyzed by peroxidase proceed via a free-radical mechanism (fig 1) immediately after the peroxidase is added to the. The effectiveness of peroxidase was measured in a varying ph environment the environment's ph can range from 1-14, 7 being neutral, 7-1 being more and more acidic towards 1, and 8-14 being more and more basic towards 14 (raven, 2011.